目的 建立蜘蛛香HPLC指纹图谱,为其质量控制提供科学依据。方法 采用Diamonsil C18色谱柱(4.6 mm×250 mm,5 μm),乙腈(A)-0.1%甲酸水溶液(B) 为流动相梯度洗脱,检测波长为327 nm(0~33 min)、256 nm(33~90 min),体积流量为1.0 mL·min-1,柱温为30 ℃。通过相似度评价、聚类分析(HCA)、主成分分析(PCA)、偏最小二乘判别分析(PLS-DA)对25批蜘蛛香指纹图谱进行分析。结果 建立了25批蜘蛛香HPLC指纹图谱,标定了36个共有峰,指认了9个共有峰,其中18批样品相似度大于0.9,所有样品可分成2类,发现缬草三酯、乙酰缬草三酯、异绿原酸A等6个差异较大的成分。结论 HPLC指纹图谱结合化学模式识别能较好地反映蜘蛛香的整体质量,可为蜘蛛香质量控制和评价提供参考。
Abstract
OBJECTIVE To establish the HPLC fingerprint of Valeriana jatamansi and provide a reference for its effective quality control. METHODS The HPLC-DAD analysis was performed on Diamonsil C18 column (4.6 mm×250 mm, 5 μm), with acetonitrile (A)-0.1% formic acid (B) solution as the mobile phase for gradient elution, the detection wavelength was set at 327 nm (0-33 min) and 256 nm (33-90 min), the flow rate was 1.0 mL·min-1, and the column temperature was maintained at 30 ℃. The fingerprints of 25 batches of Valeriana jatamansi samples were analyzed by similarity analysis, hierarchical clustering analysis (HCA), principal component analysis (PCA), and orthogonal partial least squares discriminant analysis (PLS-DA). RESULTS The fingerprints of 25 batches of Valeriana jatamansi samples were established. There were 36 common peaks in the fingerprints and nine common peaks were identified by reference substances. The fingerprints similarity of 18 batches of samples was over 0.9, and the samples were classified into two groups. Six components were the main markers that cause differences in different batches of samples, including valepotriate, acevaltrate, isochlorogenic acid A, and some others. CONCLUSION HPLC fingerprint combined with recognition of chemical pattern can reflect the intrinsic quality of Valeriana jatamansi, which may provide reference for the quality control and evaluation of Valeriana jatamansi.
关键词
蜘蛛香 /
指纹图谱 /
波长切换 /
聚类分析 /
主成分分析 /
偏最小二乘判别分析 /
化学模式识别
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Key words
Valeriana jatamansi Jones /
fingerprint /
wavelength switching /
hierarchical clustering analysis /
principal component analysis /
partial least square discriminant analysis /
chemical pattern recognition
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中图分类号：
R284
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参考文献
[1] Ch.P (2015) VolⅠ(中国药典2015年版. 一部) . 2015:368-369.
[2] YANG J, LONG Q D, LUO X R, et al. Study on extraction technology of Valeriana jatamansi Jones oil with supercritical CO2. Lishizhen Med Mater Med Res (时珍国医国药), 2012, 23(1):157-158.
[3] TIAN Y F, LONG Q D, LUO X R, et al. Gas chromatography-time of flight mass spectrometry analysis of the Valerlana jatamansi Jones oil. Lishizhen Med Mater Med Res (时珍国医国药), 2012, 23(4):924-926.
[4] LUO X R, LUO J, YANG J, et al. Determination of total valepotriates content in different parts of Valerlana jatamansi Jones . Anhui Agric Sci (安徽农业科学), 2012, 40(16):8884, 9106.
[5] LUO X R, YUAN T H, YANG J, et al. Study on supercritical CO2 extraction process of total valepotriates from Valeriana jatamansi Jones. Guangdong Agric Sci (广东农业科学), 2012, 39(16):119-121.
[6] LUO X R, YUAN T H, YANG J, et al. Optimization of supercritical CO2 method for extraction of valtrate from Valeriana jatamansi . Hubei Agric Sci (湖北农业科学), 2013, 52(8):1901-1902,1912.
[7] LI R, LI X P, WU Y. Quantitative determination of total flavones in Valeriana jatamansi Jones by UV spectrophotometry. J Liaoning Univ Tradit Chin Med (辽宁中医药大学学报), 2008, 10(12):149-150.
[8] LIU K P, YANG J, LUO X R, et al. Datermination of chlorogenic acid and total phenolic acid content in Valeriana jatarnansi . Hubei Agric Sci (湖北农业科学), 2017, 56(2):288-290.
[9] WANG F F, ZHANG Y M, WU S H, et al. Studies of the structure-antioxidant activity relationships and antioxidant activity mechanism of iridoid valepotriates and their degradation products. PLoS One, 2017, 12(12):e0189198.
[10] LIU K P, YANG J, CHENG S Y, et al. Simultaneous determination of seven components in Valeriana jatamansi by HPLC. J Chin Med Mater (中药材), 2018, 41(4):922-924.
[11] YAO C, XIN H, LU T L, et al. UPLC Fingerprint and chemical pattern recognition method of Gardeniae Fructus from different producing areas. Chin Pharm J(中国药学杂志), 2017, 52(1):63-67.
[12] ZHAO M, LONG H N, HE H, et al. Gas chromatographic fingerprinting of Valeriana jatarnansi volatile oil. Herald Med (医药导报), 2016, 35(3):291-295.
[13] GAO H L, TAN Y Z. Study on fingerprint of wild Valeriana jatarnansi in Enshi prefecture. Lishizhen Med Mater Med Res (时珍国医国药), 2013, 24(7):1749-1751.
[14] FU S H, YAO C F, ZHANG B, et al. Study on HPLC fingerprint of Valeriana jatamansi . Chin Tradit Herb Drugs (中草药), 2017, 48(21):4537-4540.
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脚注
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基金
贵州省联合基金项目资助(黔科合LH字7091号); 贵州省社发攻关项目资助(黔科合SY字3032号); 贵州省中药现代化专项项目资助(黔科合ZY字3010号)
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